Structural highlights
Publication Abstract from PubMed
Cahuitamycins are biofilm inhibitors biosynthesized by a convergent NRPS pathway. Previous genetic analysis indicated that a discrete enzyme, CahJ, serves as a gatekeeper for cahuitamycin structural diversification. Herein, the CahJ protein was probed structurally, functionally and through mutasynthesis. This analysis enabled production of a new cahuitamycin congener through targeted precursor incorporation.
A Defined and Flexible Pocket Explains Aryl Substrate Promiscuity by the Cahuitamycin Starter Unit Activating Enzyme CahJ.,Sherman DH, Tripathi A, Park SR, Sikkema A, Cho HJ, Wu J, Lee B, Xi C, Smith JL Chembiochem. 2018 May 9. doi: 10.1002/cbic.201800233. PMID:29742306[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sherman DH, Tripathi A, Park SR, Sikkema A, Cho HJ, Wu J, Lee B, Xi C, Smith JL. A Defined and Flexible Pocket Explains Aryl Substrate Promiscuity by the Cahuitamycin Starter Unit Activating Enzyme CahJ. Chembiochem. 2018 May 9. doi: 10.1002/cbic.201800233. PMID:29742306 doi:http://dx.doi.org/10.1002/cbic.201800233
