Structural highlights
1vqb is a 1 chain structure with sequence from Bpf1. This structure supersedes the now removed PDB entry 1bgh. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[G5P_BPF1] Binds to DNA in a highly cooperative manner without pronounced sequence specificity. During synthesis of the single-stranded (progeny) viral DNA, prevents the conversion into the double-stranded replicative form. G5P is displaced by the capsid protein G8P during phage assembly on the inner bacterial membrane (By similarity).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The crystal structure of the dimeric gene V protein of bacteriophage f1 was determined using multiwavelength anomalous diffraction on the selenomethionine-containing wild-type and isoleucine-47-->methionine mutant proteins with x-ray diffraction data phased to 2.5 A resolution. The structure of the wild-type protein has been refined to an R factor of 19.2% using native data to 1.8 A resolution. The structure of the gene V protein was used to obtain a model for the protein portion of the gene V protein-single-stranded DNA complex.
Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein.,Skinner MM, Zhang H, Leschnitzer DH, Guan Y, Bellamy H, Sweet RM, Gray CW, Konings RN, Wang AH, Terwilliger TC Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2071-5. PMID:8134350[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Skinner MM, Zhang H, Leschnitzer DH, Guan Y, Bellamy H, Sweet RM, Gray CW, Konings RN, Wang AH, Terwilliger TC. Structure of the gene V protein of bacteriophage f1 determined by multiwavelength x-ray diffraction on the selenomethionyl protein. Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2071-5. PMID:8134350
