| Structural highlights
6g94 is a 10 chain structure with sequence from Eco57, Ecol6 and Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , , , , , , , |
| Related: | 4gd3, 3uqy, 3usc, 3use |
| Gene: | hyaA, c1113 (ECOL6), hyaB, b0973, JW0955 (ECOLI), hyaC, Z1391, ECs1130 (ECO57) |
| Activity: | Hydrogenase (acceptor), with EC number 1.12.99.6 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[MBHS_ECOL6] This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth. [CYBH_ECO57] Probable b-type cytochrome. [MBHL_ECOLI] This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD1 is believed to have a role in hydrogen cycling during fermentative growth.
Publication Abstract from PubMed
The crystal structure of the Escherichia coli O2-sensitive C19G [NiFe]-hydrogenase-1 variant shows that the mutation results in a novel FeS cluster, proximal to the Ni-Fe active site. While the proximal cluster of the native O2-tolerant enzyme can transfer two electrons to that site, EPR spectroscopy shows that the modified cluster can transfer only one electron, this shortfall coinciding with O2 sensitivity. Computational studies on electron transfer help to explain how the structural and redox properties of the novel FeS cluster modulate the observed phenotype.
X-ray structural, functional and computational studies of the O2-sensitive E. coli hydrogenase-1 C19G variant reveal an unusual [4Fe-4S] cluster.,Volbeda A, Mouesca JM, Darnault C, Roessler MM, Parkin A, Armstrong FA, Fontecilla-Camps JC Chem Commun (Camb). 2018 Jun 11. doi: 10.1039/c8cc02896f. PMID:29888350[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Volbeda A, Mouesca JM, Darnault C, Roessler MM, Parkin A, Armstrong FA, Fontecilla-Camps JC. X-ray structural, functional and computational studies of the O2-sensitive E. coli hydrogenase-1 C19G variant reveal an unusual [4Fe-4S] cluster. Chem Commun (Camb). 2018 Jun 11. doi: 10.1039/c8cc02896f. PMID:29888350 doi:http://dx.doi.org/10.1039/c8cc02896f
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