4m3m

Publication Abstract from PubMed

A stereodivergent plan is presented leading to all eight stereoisomers of oseltamivir carboxylate (OC). Key chemical manoeuvers are (1) a three-component vinylogous Mukaiyama-Mannich reaction, which sets the whole carbon skeleton and heteroatom substituents, and (2) an intramolecular, silylative Mukaiyama aldol reaction, which creates the targeted carbocycle. The viability of the plan was demonstrated by the first total synthesis of 4-epi-oseltamivir carboxylate (), accessed in 15 steps from glyceraldehyde, o-anisidine and pyrrole siloxydiene precursors. Compound inhibits influenza A virus strains H1N1 and H3N2 at the muM level, about 150 000-fold less than the OC reference, testifying that the stereodisposition of the C4 acetamido function is key for enzyme recognition. Guided by in-depth structural evaluation including NMR solution studies, molecular mechanics simulations, docking analyses and X-ray crystallography, rationalization of the biological verdict was established.

Synthesis, structure and inhibitory activity of a stereoisomer of oseltamivir carboxylate.,Sartori A, Dell'amico L, Battistini L, Curti C, Rivara S, Pala D, Kerry PS, Pelosi G, Casiraghi G, Rassu G, Zanardi F Org Biomol Chem. 2014 Feb 11;12(10):1561-9. doi: 10.1039/c3ob42069h. PMID:24425043^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.