1aif
From Proteopedia
| |||||||
| , resolution 2.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ANTI-IDIOTYPIC FAB 409.5.3 (IGG2A) FAB FROM MOUSE
Overview
The crystal structure of anti-idiotopic Fab 409.5.3, made against an E2 specific feline infectious peritonitis virus-neutralizing antibody 730.1.4, has been determined in its free from, at 2.9 A resolution by molecular replacement. This antibody, used as an immmunogen, elicits the production of anti-anti-idiotypic antibodies that in turn neutralize the virus. The structure of the uncomplexed Fab was refined using constrained-restrained least squares minimization and simulated annealing in combination with conjugate gradient techniques to a crystallographic R of 0.22 based on 16,482 unique reflections between 20.0 and 2.9 A. The free antiidiotypic Fab shows, when compared to its complexed form, a 5 degrees rotation of its variable light with respect to its variable heavy domain and rearrangement of complementarity determining region loops, which permits optimization of the stereocomplementarity between interacting molecules. This finding supports the induced fit hypothesis for antibody antigen interaction.
About this Structure
1AIF is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structure of an anti-idiotypic Fab against feline peritonitis virus-neutralizing antibody and a comparison with the complexed Fab., Ban N, Escobar C, Hasel KW, Day J, Greenwood A, McPherson A, FASEB J. 1995 Jan;9(1):107-14. PMID:7821749
Page seeded by OCA on Sun Mar 30 18:40:52 2008
Categories: Mus musculus | Protein complex | Ban, N. | Day, J. | Escobar, C. | Greenwood, A. | Hasel, K. | McPherson, A. | C region | Immunoglobulin | V region
