Structural highlights
Function
[PCNA_PYRAB] Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication (By similarity). Regulates activity of NucS endonuclease and prevents non-specific cleavage.[HAMAP-Rule:MF_00317][1] [DP2S_PYRAB] Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
References
- ↑ Creze C, Ligabue A, Laurent S, Lestini R, Laptenok SP, Khun J, Vos MH, Czjzek M, Myllykallio H, Flament D. Modulation of the Pyrococcus abyssi NucS endonuclease activity by replication clamp at functional and structural levels. J Biol Chem. 2012 May 4;287(19):15648-60. doi: 10.1074/jbc.M112.346361. Epub 2012, Mar 19. PMID:22431731 doi:http://dx.doi.org/10.1074/jbc.M112.346361
