Structural highlights
Function
[NCAP_EBOZM] Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. During replication, encapsidation by NP is coupled to RNA synthesis and all replicative products are resistant to nucleases.
Publication Abstract from PubMed
Ebola virus is an emerging virus that is capable of causing a deadly disease in humans. Replication, transcription and packaging of the viral genome are carried out by the viral nucleocapsid. The nucleocapsid is a complex of the viral nucleoprotein, RNA and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins. The 3.1 A resolution single-particle cryo-electron microscopy structure of the nucleoprotein-RNA helical filament presented here resembles previous structures determined at lower resolution, while providing improved molecular details of protein-protein and protein-RNA interactions. The higher resolution of the structure presented here will facilitate the design and characterization of novel and specific Ebola virus therapeutics targeting the nucleocapsid.
Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex.,Kirchdoerfer RN, Saphire EO, Ward AB Acta Crystallogr F Struct Biol Commun. 2019 May 1;75(Pt 5):340-347. doi:, 10.1107/S2053230X19004424. Epub 2019 Apr 24. PMID:31045563[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kirchdoerfer RN, Saphire EO, Ward AB. Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex. Acta Crystallogr F Struct Biol Commun. 2019 May 1;75(Pt 5):340-347. doi:, 10.1107/S2053230X19004424. Epub 2019 Apr 24. PMID:31045563 doi:http://dx.doi.org/10.1107/S2053230X19004424
