5ncn

Structural highlights

Function

[MOB1_YEAST] Functions as an activator subunit for the DBF2 protein kinase. Binds to DBF2, which is required for the phosphorylation and activation of DBF2 by the upstream kinase CDC15 in late anaphase. DBF2-MOB1 is part of the mitotic exit network (MEN) signaling cascade, which regulates release from the nucleus and activity of phosphatase CDC14. Required for inactivation of mitotic cyclin-dependent kinase for exit from mitosis, cytokinesis and G1 gene transcription.^{[1] [2] [3]} [DBF2_YEAST] Ser/Thr-protein kinase involved in the mitotic exit network (MEN) and required after the metaphase to anaphase cell cycle transition. Phosphorylates CHS2 to regulate its dynamics and chitin synthesis at the division site during cytokinesis. Coordinates septin and actomyosin ring (AMR) functions during cytokinesis through the phosphorylation of HOF1. In complex with MOB1, phosphorylates CDC14 at sites adjacent to its nuclear localization sequence, thereby retaining CDC14 in the cytoplasm. Binds also to SWI5 and CLB2 mRNAs cotranscriptionally to regulate their decay. In the nucleus, the DBF2-MOB1 complex regulates passenger protein localization during anaphase. Mediates sorbic acid stress tolerance through promoting vacuolar H(+)-ATPase function, probably through phosphorylation of VMA1 and VMA2 subunits.^{[4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [22]}

References

1. ↑ Luca FC, Mody M, Kurischko C, Roof DM, Giddings TH, Winey M. Saccharomyces cerevisiae Mob1p is required for cytokinesis and mitotic exit. Mol Cell Biol. 2001 Oct;21(20):6972-83. PMID:11564880 doi:http://dx.doi.org/10.1128/MCB.21.20.6972-6983.2001
2. ↑ Mah AS, Jang J, Deshaies RJ. Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7325-30. Epub 2001 Jun 12. PMID:11404483 doi:http://dx.doi.org/10.1073/pnas.141098998
3. ↑ Stoepel J, Ottey MA, Kurischko C, Hieter P, Luca FC. The mitotic exit network Mob1p-Dbf2p kinase complex localizes to the nucleus and regulates passenger protein localization. Mol Biol Cell. 2005 Dec;16(12):5465-79. Epub 2005 Sep 21. PMID:16176976 doi:http://dx.doi.org/E05-04-0337
4. ↑ Fesquet D, Fitzpatrick PJ, Johnson AL, Kramer KM, Toyn JH, Johnston LH. A Bub2p-dependent spindle checkpoint pathway regulates the Dbf2p kinase in budding yeast. EMBO J. 1999 May 4;18(9):2424-34. doi: 10.1093/emboj/18.9.2424. PMID:10228157 doi:http://dx.doi.org/10.1093/emboj/18.9.2424
5. ↑ Frenz LM, Lee SE, Fesquet D, Johnston LH. The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis. J Cell Sci. 2000 Oct;113 Pt 19:3399-408. PMID:10984431
6. ↑ Lee SE, Frenz LM, Wells NJ, Johnson AL, Johnston LH. Order of function of the budding-yeast mitotic exit-network proteins Tem1, Cdc15, Mob1, Dbf2, and Cdc5. Curr Biol. 2001 May 15;11(10):784-8. PMID:11378390
7. ↑ Mah AS, Jang J, Deshaies RJ. Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Proc Natl Acad Sci U S A. 2001 Jun 19;98(13):7325-30. Epub 2001 Jun 12. PMID:11404483 doi:http://dx.doi.org/10.1073/pnas.141098998
8. ↑ Visintin R, Amon A. Regulation of the mitotic exit protein kinases Cdc15 and Dbf2. Mol Biol Cell. 2001 Oct;12(10):2961-74. PMID:11598184
9. ↑ Bardin AJ, Boselli MG, Amon A. Mitotic exit regulation through distinct domains within the protein kinase Cdc15. Mol Cell Biol. 2003 Jul;23(14):5018-30. PMID:12832486
10. ↑ Visintin R, Stegmeier F, Amon A. The role of the polo kinase Cdc5 in controlling Cdc14 localization. Mol Biol Cell. 2003 Nov;14(11):4486-98. Epub 2003 Aug 7. PMID:14551257 doi:http://dx.doi.org/10.1091/mbc.E03-02-0095
11. ↑ Stoepel J, Ottey MA, Kurischko C, Hieter P, Luca FC. The mitotic exit network Mob1p-Dbf2p kinase complex localizes to the nucleus and regulates passenger protein localization. Mol Biol Cell. 2005 Dec;16(12):5465-79. Epub 2005 Sep 21. PMID:16176976 doi:http://dx.doi.org/E05-04-0337
12. ↑ Mah AS, Elia AE, Devgan G, Ptacek J, Schutkowski M, Snyder M, Yaffe MB, Deshaies RJ. Substrate specificity analysis of protein kinase complex Dbf2-Mob1 by peptide library and proteome array screening. BMC Biochem. 2005 Oct 21;6:22. doi: 10.1186/1471-2091-6-22. PMID:16242037 doi:http://dx.doi.org/10.1186/1471-2091-6-22
13. ↑ Makrantoni V, Dennison P, Stark MJ, Coote PJ. A novel role for the yeast protein kinase Dbf2p in vacuolar H+-ATPase function and sorbic acid stress tolerance. Microbiology. 2007 Dec;153(Pt 12):4016-26. doi: 10.1099/mic.0.2007/010298-0. PMID:18048916 doi:http://dx.doi.org/10.1099/mic.0.2007/010298-0
14. ↑ Mohl DA, Huddleston MJ, Collingwood TS, Annan RS, Deshaies RJ. Dbf2-Mob1 drives relocalization of protein phosphatase Cdc14 to the cytoplasm during exit from mitosis. J Cell Biol. 2009 Feb 23;184(4):527-39. doi: 10.1083/jcb.200812022. Epub 2009 Feb, 16. PMID:19221193 doi:http://dx.doi.org/10.1083/jcb.200812022
15. ↑ Konig C, Maekawa H, Schiebel E. Mutual regulation of cyclin-dependent kinase and the mitotic exit network. J Cell Biol. 2010 Feb 8;188(3):351-68. doi: 10.1083/jcb.200911128. Epub 2010 Feb , 1. PMID:20123997 doi:http://dx.doi.org/10.1083/jcb.200911128
16. ↑ Meitinger F, Petrova B, Lombardi IM, Bertazzi DT, Hub B, Zentgraf H, Pereira G. Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast. J Cell Sci. 2010 Jun 1;123(Pt 11):1851-61. doi: 10.1242/jcs.063891. Epub 2010 May, 4. PMID:20442249 doi:http://dx.doi.org/10.1242/jcs.063891
17. ↑ Trcek T, Larson DR, Moldon A, Query CC, Singer RH. Single-molecule mRNA decay measurements reveal promoter- regulated mRNA stability in yeast. Cell. 2011 Dec 23;147(7):1484-97. doi: 10.1016/j.cell.2011.11.051. PMID:22196726 doi:http://dx.doi.org/10.1016/j.cell.2011.11.051
18. ↑ Oh Y, Chang KJ, Orlean P, Wloka C, Deshaies R, Bi E. Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol Biol Cell. 2012 Jul;23(13):2445-56. doi: 10.1091/mbc.E12-01-0033. Epub 2012, May 9. PMID:22573892 doi:http://dx.doi.org/10.1091/mbc.E12-01-0033
19. ↑ Meitinger F, Palani S, Hub B, Pereira G. Dual function of the NDR-kinase Dbf2 in the regulation of the F-BAR protein Hof1 during cytokinesis. Mol Biol Cell. 2013 May;24(9):1290-304. doi: 10.1091/mbc.E12-08-0608. Epub 2013, Feb 27. PMID:23447700 doi:http://dx.doi.org/10.1091/mbc.E12-08-0608
20. ↑ Toyn JH, Johnston LH. The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition. EMBO J. 1994 Mar 1;13(5):1103-13. PMID:8131744
21. ↑ Komarnitsky SI, Chiang YC, Luca FC, Chen J, Toyn JH, Winey M, Johnston LH, Denis CL. DBF2 protein kinase binds to and acts through the cell cycle-regulated MOB1 protein. Mol Cell Biol. 1998 Apr;18(4):2100-7. PMID:9528782
22. ↑ Grandin N, de Almeida A, Charbonneau M. The Cdc14 phosphatase is functionally associated with the Dbf2 protein kinase in Saccharomyces cerevisiae. Mol Gen Genet. 1998 Apr;258(1-2):104-16. PMID:9613578