5hj0
From Proteopedia
Crystal Structure of Mis18 'Yippee-like' Domain
Structural highlights
Function[MIS18_SCHPO] Required for correct chromosome segregation where it has a role in the formation and/or maintenance of specialized chromatin at the centromere. Has a role in kinetochore assembly and CENP-A loading. Maintains the deacetylated state of histones specifically in the central core of the centromeres.[1] Publication Abstract from PubMedMis18 is a key regulator responsible for the centromere localization of the CENP-A chaperone Scm3 in Schizosaccharomyces pombe and HJURP in humans, which establishes CENP-A chromatin that defines centromeres. The molecular and structural determinants of Mis18 centromere targeting remain elusive. Here, by combining structural, biochemical, and yeast genetic studies, we show that the oligomerization of S. pombe Mis18, mediated via its conserved N-terminal Yippee-like domain, is crucial for its centromere localization and function. The crystal structure of the N-terminal Yippee-like domain reveals a fold containing a cradle-shaped pocket that is implicated in protein/nucleic acid binding, which we show is required for Mis18 function. While the N-terminal Yippee-like domain forms a homodimer in vitro and in vivo, full-length Mis18, including the C-terminal alpha-helical domain, forms a homotetramer in vitro. We also show that the Yippee-like domains of human Mis18alpha/Mis18beta interact to form a heterodimer, implying a conserved structural theme for Mis18 regulation. Centromere localization and function of Mis18 requires Yippee-like domain-mediated oligomerization.,Subramanian L, Medina-Pritchard B, Barton R, Spiller F, Kulasegaran-Shylini R, Radaviciute G, Allshire RC, Arockia Jeyaprakash A EMBO Rep. 2016 Feb 26. pii: e201541520. PMID:26921242[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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