5j5c

Structural highlights

Function

[ARL1_HUMAN] GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus.^[1] [BIG1_HUMAN] Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined.^{[2] [3] [4] [5] [6]}

References

1. ↑ Hong JX, Lee FJ, Patton WA, Lin CY, Moss J, Vaughan M. Phospholipid- and GTP-dependent activation of cholera toxin and phospholipase D by human ADP-ribosylation factor-like protein 1 (HARL1). J Biol Chem. 1998 Jun 19;273(25):15872-6. PMID:9624189
2. ↑ Li H, Adamik R, Pacheco-Rodriguez G, Moss J, Vaughan M. Protein kinase A-anchoring (AKAP) domains in brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2). Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1627-32. Epub 2003 Feb 5. PMID:12571360 doi:10.1073/pnas.0337678100
3. ↑ Saeki N, Tokuo H, Ikebe M. BIG1 is a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity. J Biol Chem. 2005 Mar 18;280(11):10128-34. Epub 2005 Jan 11. PMID:15644318 doi:10.1074/jbc.M413415200
4. ↑ Shen X, Hong MS, Moss J, Vaughan M. BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein, is required for correct glycosylation and function of integrin beta1. Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1230-5. Epub 2007 Jan 16. PMID:17227842 doi:10.1073/pnas.0610535104
5. ↑ Li CC, Kuo JC, Waterman CM, Kiyama R, Moss J, Vaughan M. Effects of brefeldin A-inhibited guanine nucleotide-exchange (BIG) 1 and KANK1 proteins on cell polarity and directed migration during wound healing. Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19228-33. doi:, 10.1073/pnas.1117011108. Epub 2011 Nov 14. PMID:22084092 doi:10.1073/pnas.1117011108
6. ↑ Boal F, Stephens DJ. Specific functions of BIG1 and BIG2 in endomembrane organization. PLoS One. 2010 Mar 25;5(3):e9898. doi: 10.1371/journal.pone.0009898. PMID:20360857 doi:10.1371/journal.pone.0009898