Methane monooxygenase

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spinqualitylabelsall models Structure of methane monooxygenase α (grey and green) +β (pink and yellow) +γ (magenta and cyan) subunits complex with Fe+3 (red), Ca+2 (green) and dibromomethane (PDB code 1fz8). Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Function Methane monooxygenase (MMO) catalyzes the oxidation of the C-H bond of methane and other alkanes. MMO catalyzes the conversion of CH4 and O2 to CH3OH and H2O using NADPH as reducing agent. The soluble MMO (sMMO) has Fe-O-Fe in its active site[1] while the particulate MMO (pMMO) has Cu in it[2]. MMO is composed of 3 subunits. Subunit α is the hydroxylase (MMOH) and contains the di-iron active site. Subunit β is a reductase which contains the co-factors FAD and 2Fe-2S complex. Subunit γ is regulatory (MMOB) and is involved in coupling. MMO is found in methanotropic bacteria.

3D structures of methane monooxygenase

Updated on 06-September-2020 {{#tree:id=OrganizedByTopic|openlevels=0|

• Methane monooxygenase

• • 1mmo, 1mty, 1fyz, 1fz0, 1fz1, 1fz2, 1fz3, 1fz4, 1fz5, 1fz6, 1fz7, 1fzh, 1fzi, 1xu5 – McMMO α+β+γ subunits + Fe – Methylococcus capsulatus
    
  • 1xmf – McMMO α+β+γ subunits + Mn
    
  • 1xmh – McMMO α+β+γ subunits + Co
    
  • 1xmg – McMMO α+β+γ subunits
    
  • 1yew, 3rgb – McMMO α+β+γ subunits + Cu + Zn
    
  • 1mhy, 1mhz, 6vk5, 6vk6, [[6vk7] – MtMMO α+β+γ subunits + Fe+3 – Methylosinus trichosporium
    
  • 6vk4 – MtMMO α+β+γ subunits + Fe+3 + Fe+2
    
  • 6vk8 – MtMMO α+β+γ subunits + Fe+3 + succinate
    
  • 6cxh – MMO α+β+γ subunits + Cu – Methylomicrobium alcaliphilum
    

• MMO complexes

• • 1fz8 – McMMO α+β+γ subunits + Fe + dibromomethane
    
  • 1xvc – McMMO α+β+γ subunits + Fe + bromoethane + bromopentane
    
  • 1xvb – McMMO α+β+γ subunits + Fe + bromoethane + bromopropane + bromobutane + bromohexanol
    
  • 1fz9 – McMMO α+β+γ subunits + Fe + iodoethane
    
  • 1xvd – McMMO α+β+γ subunits + Fe + fluorophenol
    
  • 1xve – McMMO α+β+γ subunits + Fe + bromobutenol
    
  • 1xvf – McMMO α+β+γ subunits + Fe + chloropropanol
    
  • 1xvg – McMMO α+β+γ subunits + Fe + bromoethanol
    
  • 1xu3 – McMMO α+β+γ subunits + Fe + bromophenol
    
  • 4gam – McMMO α+β+γ subunits + Fe + MMO regulatory protein B
    
  • 4phz, 4pi0 – MeMMO α+β+γ subunits + Cu + peptide - methylocystis
    
  • 4pi2 – MeMMO α+β+γ subunits + Cu + Zn + peptide
    

• MMO γ subunit (MMO reductase, MMOR)

• • 1jq4 – McMMOR N terminal + Fe2S2 - NMR
    
  • 1tvc – McMMOR C terminal + FAD derivative - NMR
    

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References

1. ↑ Lipscomb JD. Biochemistry of the soluble methane monooxygenase. Annu Rev Microbiol. 1994;48:371-99. PMID:7826011 doi:http://dx.doi.org/10.1146/annurev.mi.48.100194.002103
2. ↑ Miyaji A. Particulate methane monooxygenase from Methylosinus trichosporium OB3b. Methods Enzymol. 2011;495:211-25. doi: 10.1016/B978-0-12-386905-0.00014-0. PMID:21419924 doi:http://dx.doi.org/10.1016/B978-0-12-386905-0.00014-0