6st9

Publication Abstract from PubMed

Although strawberries are highly appreciated fruits, their intake can induce allergic reactions in atopic patients. These reactions can be due to the patient s previous sensitization to the major birch-pollen allergen Bet v 1, by which, IgE generated in response to Bet v 1 crossreacts with the structurally-related strawberry Fra a 1 protein family. Fra a 1.02 is the most expressed paralog in ripe strawberries and is highly allergenic. To better understand the molecular mechanisms regulating this allergic response, we have determined the three-dimensional structure of Fra a 1.02 and four site-directed mutants that were designed based on their positions in potential epitopes. Fra a 1.02 and mutants conform to the START fold. We show that the cross-reactivity of all the mutant variants to IgE from patients allergic to Bet v 1 was significantly reduced without altering the conserved structural fold, so that they could potentially be used as hypoallergenic Fra a 1 variants for the generation of vaccines against strawberry allergy in atopic patients.

STRUCTURAL BASIS FOR THE ALLERGENICITY OF Fra a 1.02 IN STRAWBERRY FRUITS.,Orozco-Navarrete B, Kaczmarska Z, Dupeux F, Garrido-Aranda M, Pott D, Diaz-Perales A, Casanal A, Marquez JA, Valpuesta V, Merchante C J Agric Food Chem. 2019 Nov 27. doi: 10.1021/acs.jafc.9b05714. PMID:31774998^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.