Structural highlights
Publication Abstract from PubMed
Potassium ion channels utilize a highly selective filter to rapidly transport K(+) ions across cellular membranes. This selectivity filter is composed of four binding sites which display almost equal electron density in crystal structures with high potassium ion concentrations. This electron density can be interpreted to reflect a superposition of alternating potassium ion and water occupied states or as adjacent potassium ions. Here, we use single wavelength anomalous dispersion (SAD) X-ray diffraction data collected near the potassium absorption edge to show experimentally that all ion binding sites within the selectivity filter are fully occupied by K(+) ions. These data support the hypothesis that potassium ion transport occurs by direct Coulomb knock-on, and provide an example of solving the phase problem by K-SAD.
Anomalous X-ray diffraction studies of ion transport in K(+) channels.,Langan PS, Vandavasi VG, Weiss KL, Afonine PV, El Omari K, Duman R, Wagner A, Coates L Nat Commun. 2018 Oct 31;9(1):4540. doi: 10.1038/s41467-018-06957-w. PMID:30382100[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Langan PS, Vandavasi VG, Weiss KL, Afonine PV, El Omari K, Duman R, Wagner A, Coates L. Anomalous X-ray diffraction studies of ion transport in K(+) channels. Nat Commun. 2018 Oct 31;9(1):4540. doi: 10.1038/s41467-018-06957-w. PMID:30382100 doi:http://dx.doi.org/10.1038/s41467-018-06957-w
