Structural highlights
Function
[MUG_ECOLI] Excises ethenocytosine and uracil, which can arise by alkylation or deamination of cytosine, respectively, from the corresponding mispairs with guanine in ds-DNA. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Base-excision of a self-complementary oligonucleotide with central G:T mismatches by the G:T/U-specific mismatch DNA glycosylase (MUG), generates an unusual DNA structure which is remarkably similar in conformation to an interstrand DNA adduct of the anti-tumor drug cis-diamminedichloroplatinum. The abasic sugars generated by excision of the mismatched thymines are extruded from the double-helix, and the 'widowed' deoxyguanosines rotate so that their N7 and O6 groups protrude into the minor groove of the duplex and restack in an interleaved intercalative geometry, generating a kink in the helix axis.
Structure of a DNA base-excision product resembling a cisplatin inter-strand adduct.,Barrett TE, Savva R, Barlow T, Brown T, Jiricny J, Pearl LH Nat Struct Biol. 1998 Aug;5(8):697-701. PMID:9699633[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gallinari P, Jiricny J. A new class of uracil-DNA glycosylases related to human thymine-DNA glycosylase. Nature. 1996 Oct 24;383(6602):735-8. PMID:8878487 doi:http://dx.doi.org/10.1038/383735a0
- ↑ O'Neill RJ, Vorob'eva OV, Shahbakhti H, Zmuda E, Bhagwat AS, Baldwin GS. Mismatch uracil glycosylase from Escherichia coli: a general mismatch or a specific DNA glycosylase? J Biol Chem. 2003 Jun 6;278(23):20526-32. Epub 2003 Mar 31. PMID:12668677 doi:http://dx.doi.org/10.1074/jbc.M210860200
- ↑ Barrett TE, Savva R, Barlow T, Brown T, Jiricny J, Pearl LH. Structure of a DNA base-excision product resembling a cisplatin inter-strand adduct. Nat Struct Biol. 1998 Aug;5(8):697-701. PMID:9699633 doi:10.1038/1394
