2frh
From Proteopedia
Crystal Structure of Sara, A Transcription Regulator From Staphylococcus Aureus
Structural highlights
Function[SARA_STAAW] Global regulator with both positive and negative effects that controls the expression of several virulence factors and the biofilm formation process in a cell density-dependent manner (By similarity). Required for transcription of primary transcripts RNAII and RNAIII generated by agr (virulence accessory gene regulator) locus. Negatively regulates the expression of spa (protein A) and aur (metalloprotease aureolysin). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe sarA locus in Staphylococcus aureus controls the expression of many virulence genes. The sarA regulatory molecule, SarA, is a 14.7-kDa protein (124 residues) that binds to the promoter region of target genes. Here we report the 2.6 A-resolution x-ray crystal structure of the dimeric winged helix SarA protein, which differs from the published SarA structure dramatically. In the crystal packing, multiple dimers of SarA form a scaffold, possibly via divalent cations. Mutations of individual residues within the DNA-binding helix-turn-helix and the winged region as well as within the metal-binding pocket implicate basic residues R84 and R90 within the winged region to be critical in DNA binding, whereas acidic residues D88 and E89 (wing), D8 and E11 (metal-binding pocket), and cysteine 9 are essential for SarA function. These data suggest that the winged region of the winged helix protein participates in DNA binding and activation, whereas the putative divalent cation binding pocket is only involved in gene function. Structural and function analyses of the global regulatory protein SarA from Staphylococcus aureus.,Liu Y, Manna AC, Pan CH, Kriksunov IA, Thiel DJ, Cheung AL, Zhang G Proc Natl Acad Sci U S A. 2006 Feb 14;103(7):2392-7. Epub 2006 Feb 2. PMID:16455801[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
| ||||||||||||||||||||||
