Structural highlights
Function
[TFC8_YEAST] TFIIIC mediates tRNA and 5S RNA gene activation by binding to intragenic promoter elements. Upstream of the transcription start site, TFIIIC assembles the initiation complex TFIIIB-TFIIIC-tDNA, which is sufficient for RNA polymerase III recruitment and function. Part of the tauB domain of TFIIIC that binds boxB DNA promoter sites of tRNA and similar genes. Plays a role in TFIIB assembly through its interaction with SPT15/TBP. Essential for cell viability.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Yeast RNA polymerase III is recruited upon binding of subcomplexes tauA and tauB of transcription factor IIIC (TFIIIC) to the A and B blocks of tRNA gene promoters. The tauB subcomplex consists of subunits tau60, tau91, and tau138. We determined the 3.2 A crystal structure of tau60 bound to a large C-terminal fragment of tau91 (Deltatau91). Deltatau91 protein contains a seven-bladed propeller preceded by an N-terminal extension, whereas tau60 contains a structurally homologous propeller followed by a C-terminal domain with a novel alpha/beta fold. The two propeller domains do not have any detectable DNA binding activity and mediate heterodimer formation that may serve as scaffold for tau138 assembly. We show that the C-terminal tau60 domain interacts with the TATA binding protein (TBP). Recombinant tauB recruits TBP and stimulates TFIIIB-directed transcription on a TATA box containing tRNA gene, implying a combined contribution of tauA and tauB to preinitiation complex formation.
Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly.,Mylona A, Fernandez-Tornero C, Legrand P, Haupt M, Sentenac A, Acker J, Muller CW Mol Cell. 2006 Oct 20;24(2):221-32. PMID:17052456[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Deprez E, Arrebola R, Conesa C, Sentenac A. A subunit of yeast TFIIIC participates in the recruitment of TATA-binding protein. Mol Cell Biol. 1999 Dec;19(12):8042-51. PMID:10567530
- ↑ Mylona A, Fernandez-Tornero C, Legrand P, Haupt M, Sentenac A, Acker J, Muller CW. Structure of the tau60/Delta tau91 subcomplex of yeast transcription factor IIIC: insights into preinitiation complex assembly. Mol Cell. 2006 Oct 20;24(2):221-32. PMID:17052456 doi:10.1016/j.molcel.2006.08.013
