Structural highlights
2qxf is a 1 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , |
| Gene: | sbcB, cpeA, xonA, b2011, JW1993 (ECOLI) |
| Activity: | Exodeoxyribonuclease I, with EC number 3.1.11.1 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[EX1_ECOLI] Also functions as a DNA deoxyribophosphodiesterase that releases deoxyribose-phosphate moieties following the cleavage DNA at an apurinic/apyrimidinic (AP) site by either an AP endonuclease AP lyase.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
In Escherichia coli, exonuclease I (ExoI) is a monomeric processive 3'-5' exonuclease that degrades single-stranded DNA. The enzyme has been implicated as primarily being involved in repairing frameshift mutations. The structure of the enzyme has previously been determined in a hexagonal space group at 2.4 A resolution. Here, the structure of ExoI in complex with a nucleotide product, thymidine 5'-monophosphate, is described in an orthorhombic space group at 1.5 A resolution. This new high-resolution structure provides some insight into the interactions involved in binding a nucleotide product. The conserved active site contains a unique metal-binding position when compared with orthologous sites in the Klenow fragment, T4 DNA polymerase and dnaQ. This unique difference is proposed to be a consequence of the repositioning of an important histidine, His181, away from the active site.
Structure of Escherichia coli exonuclease I in complex with thymidine 5'-monophosphate.,Busam RD Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):206-10. Epub 2008, Jan 16. PMID:18219121[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Busam RD. Structure of Escherichia coli exonuclease I in complex with thymidine 5'-monophosphate. Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):206-10. Epub 2008, Jan 16. PMID:18219121 doi:10.1107/S090744490706012X
