Transcription-repair coupling factor

From Proteopedia

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The bacterial transcription-repair coupling factor TRCF, also called Mfd translocase, is a DNA repair protein. It has a role in transcription-coupled repair, a subpathway of nucleotide excision repair (NER). Mfd recognizes stalled RNA polymerase and either restarts transcription or removes the stalled polymerase and recruits the NER proteins UvrA and UvrB.

Contents 1 Function 2 Structural highlights 3 3D Structures of Transcription-repair coupling factor 4 Reference

Function

Transcription-repair coupling factor (TRCF) or Mfd enables the coupling of these processes in bacteria and humans. The TRCF has ATPase activity.

Size exclusion chromatogaphy indicates that the protein

Structural highlights

spinqualitylabelsall models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image an N→C rainbow similar to Figure 1A of the article describing the structure. . The domains in the structure of Mfd: similar to Figure 1B and Figure 2 of the article describing the structure. Figure 1B and Figure 2 of the article describing the structure. . [Note: the following view generates a substantial surface which may take several minutes to calculate. Use the one above as an alternative unless you are willing to spend the time.] Figure 1B. displayed as in Figure 3A of the article describing the structure. are highlighted in red. See figure 3B. displayed similar to Figure 3B of the article describing the structure. indicated similar to Figure 3C of the article describing the structure.

3D Structures of Transcription-repair coupling factor

Updated on 15-August-2021

2eyq – EcTRCF – Escherichia coli
6X2N, 6X2F, 6X26, 6X50, 6X43, 6X4W, 6X4Y - EcTRCF, RNA polymerase, RNA, DNA (Cryo EM) /> 3hjh – EcTRCF residues 1-470
2b2n - EcTRCF residues 1-333
6yhz - EcTRCF residues 472-547 – NMR
4dfc – EcTRCF D2 domain 127-213 + UvrABC system protein A
6xeo – EcTRCF + DNA – Cryo EM
3mlq – TtTRCF RNA polymerase interacting domain + DNA-directed RNA polymerase subunit β - Thermus thermophilus
6m6a – TtTRCF + RNA polymerase – Cryo EM
6m6b – TtTRCF + RNA polymerase + ATP-γ-S – Cryo EM
2qsr – TRCF C terminal – Streptococcus pneumoni
6ac6, 6aca, 6ac8 – MsTRCF – Mycobacterium smegmatis
6acx – MsTRCF + ADP

Reference

• Deaconescu AM, Chambers AL, Smith AJ, Nickels BE, Hochschild A, Savery NJ, Darst SA. Structural basis for bacterial transcription-coupled DNA repair. Cell. 2006 Feb 10;124(3):507-20. PMID:16469698 doi:10.1016/j.cell.2005.11.045

Created with the participation of Wayne Decatur