Structural highlights
Function
[PEX13_YEAST] Component of the peroxisomal translocation machinery with PEX14 and PEX17. Interacts with the PTS1 receptor (PAS10/PEX5).
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Pex13p is an essential component of the peroxisomal protein import machinery and interacts via its C-terminal SH3 domain with the type II SH3-ligand Pex14p and the non-PXXP protein Pex5p. We report the solution structure of the SH3 domain of Pex13p from Saccharomyces cerevisiae and the identification of a novel-binding pocket, which binds a non-PXXP-peptide representing the binding site of Pex5p. Chemical shift assays revealed the binding sites for Pex5p and Pex14p ligand peptides to be distinct and spatially separated. Competition assays demonstrated that the two ligand peptides can bind simultaneously to the SH3 domain.
The ScPex13p SH3 domain exposes two distinct binding sites for Pex5p and Pex14p.,Pires JR, Hong X, Brockmann C, Volkmer-Engert R, Schneider-Mergener J, Oschkinat H, Erdmann R J Mol Biol. 2003 Mar 7;326(5):1427-35. PMID:12595255[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pires JR, Hong X, Brockmann C, Volkmer-Engert R, Schneider-Mergener J, Oschkinat H, Erdmann R. The ScPex13p SH3 domain exposes two distinct binding sites for Pex5p and Pex14p. J Mol Biol. 2003 Mar 7;326(5):1427-35. PMID:12595255
