Structural highlights
3m0e is a 7 chain structure with sequence from "aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , |
| Related: | 1ny6, 1ny5, 1zy2 |
| Gene: | aq_1117, ntrC1 ("Aquifex aeolicus" Huber and Stetter 2001) |
| Activity: | Adenosinetriphosphatase, with EC number 3.6.1.3 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
The NtrC-like AAA+ ATPases control virulence and other important bacterial activities through delivering mechanical work to sigma54-RNA polymerase to activate transcription from sigma54-dependent genes. We report the first crystal structure for such an ATPase, NtrC1 of Aquifex aeolicus, in which the catalytic arginine engages the gamma-phosphate of ATP. Comparing the new structure with those previously known for apo and ADP-bound states supports a rigid-body displacement model that is consistent with large-scale conformational changes observed by low-resolution methods. First, the arginine finger induces rigid-body roll, extending surface loops above the plane of the ATPase ring to bind sigma54. Second, ATP hydrolysis permits Pi release and retraction of the arginine with a reversed roll, remodeling sigma54-RNAP. This model provides a fresh perspective on how ATPase subunits interact within the ring-ensemble to promote transcription, directing attention to structural changes on the arginine-finger side of an ATP-bound interface.
Engagement of arginine finger to ATP triggers large conformational changes in NtrC1 AAA+ ATPase for remodeling bacterial RNA polymerase.,Chen B, Sysoeva TA, Chowdhury S, Guo L, De Carlo S, Hanson JA, Yang H, Nixon BT Structure. 2010 Nov 10;18(11):1420-30. PMID:21070941[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chen B, Sysoeva TA, Chowdhury S, Guo L, De Carlo S, Hanson JA, Yang H, Nixon BT. Engagement of arginine finger to ATP triggers large conformational changes in NtrC1 AAA+ ATPase for remodeling bacterial RNA polymerase. Structure. 2010 Nov 10;18(11):1420-30. PMID:21070941 doi:10.1016/j.str.2010.08.018
