| Structural highlights
Function
[CTBP1_RAT] Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity. Functions in brown adipose tissue (BAT) differentiation.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
C-terminal binding proteins (CtBPs) are multi-functional proteins involved in nuclear transcriptional co-repression, Golgi membrane fission, and synaptic ribbon formation. Binding of NAD(H) to CtBPs promotes dimerization. CtBP dimers act as a scaffold for multimeric protein complex formation, thus bridging transcriptional repressors and their targets in the nucleus. Based on size-exclusion chromatography experiments and on the crystal structure of the NAD(H)-free G172E CtBP mutant, we show here that absence of NAD(H) induces flexibility/backbone conformational changes at the dimerization interface and at the CtBP interdomain region. The results presented shed first light on the correlation between NAD(H)-binding and functional CtBP dimerization.
CtBP1/BARS Gly172-->Glu mutant structure: impairing NAD(H)-binding and dimerization.,Nardini M, Valente C, Ricagno S, Luini A, Corda D, Bolognesi M Biochem Biophys Res Commun. 2009 Mar 27;381(1):70-4. Epub 2009 Feb 10. PMID:19351597[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Spano S, Silletta MG, Colanzi A, Alberti S, Fiucci G, Valente C, Fusella A, Salmona M, Mironov A, Luini A, Corda D. Molecular cloning and functional characterization of brefeldin A-ADP-ribosylated substrate. A novel protein involved in the maintenance of the Golgi structure. J Biol Chem. 1999 Jun 18;274(25):17705-10. PMID:10364211
- ↑ Di Girolamo M, Silletta MG, De Matteis MA, Braca A, Colanzi A, Pawlak D, Rasenick MM, Luini A, Corda D. Evidence that the 50-kDa substrate of brefeldin A-dependent ADP-ribosylation binds GTP and is modulated by the G-protein beta gamma subunit complex. Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):7065-9. PMID:7624370
- ↑ Nardini M, Spano S, Cericola C, Pesce A, Massaro A, Millo E, Luini A, Corda D, Bolognesi M. CtBP/BARS: a dual-function protein involved in transcription co-repression and Golgi membrane fission. EMBO J. 2003 Jun 16;22(12):3122-30. PMID:12805226 doi:http://dx.doi.org/10.1093/emboj/cdg283
- ↑ Nardini M, Valente C, Ricagno S, Luini A, Corda D, Bolognesi M. CtBP1/BARS Gly172-->Glu mutant structure: impairing NAD(H)-binding and dimerization. Biochem Biophys Res Commun. 2009 Mar 27;381(1):70-4. Epub 2009 Feb 10. PMID:19351597 doi:10.1016/j.bbrc.2009.02.010
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