Structural highlights
Publication Abstract from PubMed
Three-dimensional cryomaps have been reconstructed for tRNA-ribosome complexes in pre- and posttranslocational states at 17-A resolution. The positions of tRNAs in the A and P sites in the pretranslocational complexes and in the P and E sites in the posttranslocational complexes have been determined. Of these, the P-site tRNA position is the same as seen earlier in the initiation-like fMet-tRNA(f)(Met)-ribosome complex, where it was visualized with high accuracy. Now, the positions of the A- and E-site tRNAs are determined with similar accuracy. The positions of the CCA end of the tRNAs at the A site are different before and after peptide bond formation. The relative positions of anticodons of P- and E-site tRNAs in the posttranslocational state are such that a codon-anticodon interaction at the E site appears feasible.
Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle.,Agrawal RK, Spahn CM, Penczek P, Grassucci RA, Nierhaus KH, Frank J J Cell Biol. 2000 Aug 7;150(3):447-60. PMID:10931859[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Agrawal RK, Spahn CM, Penczek P, Grassucci RA, Nierhaus KH, Frank J. Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle. J Cell Biol. 2000 Aug 7;150(3):447-60. PMID:10931859
