Structural highlights
Function
[GLCT_BACSU] Mediates the positive regulation of the glucose PTS operon (ptsGHI) by functioning as an antiterminator factor of transcription via its interaction with the RNA-antiterminator (RAT) sequence located upstream of the ptsG gene. The RNA-binding domain of GlcT directly binds to the RNA antiterminator (RAT) sequence and prevents transcriptional termination. GlcT binding requires two identical and nearly symmetrical triple base pairings in the RAT sequence.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Ab initio macromolecular phasing has been so far limited to small proteins diffracting at atomic resolution (beyond 1.2 A) unless heavy atoms are present. We describe a general ab initio phasing method for 2 A data, based on combination of localizing model fragments such as small a-helices with Phaser and density modification with SHELXE. We implemented this approach in the program Arcimboldo to solve a 222-amino-acid structure at 1.95 A.
Crystallographic ab initio protein structure solution below atomic resolution.,Rodriguez DD, Grosse C, Himmel S, Gonzalez C, de Ilarduya IM, Becker S, Sheldrick GM, Uson I Nat Methods. 2009 Sep;6(9):651-3. Epub 2009 Aug 16. PMID:19684596[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Stulke J, Martin-Verstraete I, Zagorec M, Rose M, Klier A, Rapoport G. Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT. Mol Microbiol. 1997 Jul;25(1):65-78. PMID:11902727
- ↑ Bachem S, Stulke J. Regulation of the Bacillus subtilis GlcT antiterminator protein by components of the phosphotransferase system. J Bacteriol. 1998 Oct;180(20):5319-26. PMID:9765562
- ↑ Rodriguez DD, Grosse C, Himmel S, Gonzalez C, de Ilarduya IM, Becker S, Sheldrick GM, Uson I. Crystallographic ab initio protein structure solution below atomic resolution. Nat Methods. 2009 Sep;6(9):651-3. Epub 2009 Aug 16. PMID:19684596 doi:10.1038/nmeth.1365
