1h21

From Proteopedia

Revision as of 17:56, 30 March 2008 by OCA (Talk | contribs)
Jump to: navigation, search


PDB ID 1h21

Drag the structure with the mouse to rotate
, resolution 2.50Å
Sites:
Ligands:
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



A NOVEL IRON CENTRE IN THE SPLIT-SORET CYTOCHROME C FROM DESULFOVIBRIO DESULFURICANS ATCC 27774


Overview

The facultative sulfate/nitrate-reducing bacterium Desulfovibrio desulfuricans ATCC 27774 harbours a split-Soret cytochrome c. This cytochrome is a homodimeric protein, having two bis-histidinyl c-type haems per monomer. It has an unique architecture at the haem domain: each haem has one of the coordinating histidines provided by the other monomer, and in each monomer the haems are parallel to each other, almost in van der Waals contact. This work reports the cloning and sequencing of the gene encoding for this cytochrome and shows, by transcriptional analysis, that it is more expressed in nitrate-grown cells than in sulfate-grown ones. In addition, the gene-deduced amino acid sequence revealed two new cysteine residues that could be involved in the binding of a non-haem iron centre. Indeed, the presence of a novel type of an iron-sulfur centre (possibly of the [2Fe-2S] type) was demonstrated by EPR spectroscopy, and putative models for its localization and structure in the cytochrome molecule are proposed on the basis of the so-far-known 3D crystallographic structure of the aerobically purified split-Soret cytochrome, which lacks this centre.

About this Structure

1H21 is a Single protein structure of sequence from Desulfovibrio desulfuricans. This structure supersedes the now removed PDB entry 1DDC. Full crystallographic information is available from OCA.

Reference

A novel iron centre in the split-Soret cytochrome c from Desulfovibrio desulfuricans ATCC 27774., Abreu IA, Lourenco AI, Xavier AV, LeGall J, Coelho AV, Matias PM, Pinto DM, Armenia Carrondo M, Teixeira M, Saraiva LM, J Biol Inorg Chem. 2003 Feb;8(3):360-70. Epub 2002 Dec 19. PMID:12589573

Page seeded by OCA on Sun Mar 30 20:56:03 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools