3o15

Structural highlights

Function

[THIE_BACSU] Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP). Is also able to use the 2-methoxy analog MeO-HMP-PP, as substrate in vitro, but not the 2-trifluoromethyl analog CF(3)-HMP-PP.^{[1] [2]}

References

1. ↑ Zhang Y, Taylor SV, Chiu HJ, Begley TP. Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis. J Bacteriol. 1997 May;179(9):3030-5. PMID:9139923
2. ↑ Reddick JJ, Nicewonger R, Begley TP. Mechanistic studies on thiamin phosphate synthase: evidence for a dissociative mechanism. Biochemistry. 2001 Aug 28;40(34):10095-102. PMID:11513588