Structural highlights
Function
[ARAR_BACSU] Transcriptional repressor of the arabinose utilization genes. Also regulates its own expression. Binds to two sequences within the promoters of the araABDLMNPQ-abfA operon and the araE gene, and to one sequence in the araR promoter.
Publication Abstract from PubMed
In Bacillus subtilis, the arabinose repressor AraR negatively controls the expression of genes in the metabolic pathway of arabinose-containing polysaccharides. The protein is composed of two domains of different phylogenetic origin and function: an N-terminal DNA-binding domain belonging to the GntR family and a C-terminal effector-binding domain that shows similarity to members of the GalR/LacI family. The crystal structure of the C-terminal effector-binding domain of AraR in complex with the effector L-arabinose has been determined at 2.2 A resolution. The L-arabinose binding affinity was characterized by isothermal titration calorimetry and differential scanning fluorimetry; the K(d) value was 8.4 +/- 0.4 microM. The effect of L-arabinose on the protein oligomeric state was investigated in solution and detailed analysis of the crystal identified a dimer organization which is distinctive from that of other members of the GalR/LacI family.
Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis.,Prochazkova K, Cermakova K, Pachl P, Sieglova I, Fabry M, Otwinowski Z, Rezacova P Acta Crystallogr D Biol Crystallogr. 2012 Feb;68(Pt 2):176-85. Epub 2012 Jan 17. PMID:22281747[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Prochazkova K, Cermakova K, Pachl P, Sieglova I, Fabry M, Otwinowski Z, Rezacova P. Structure of the effector-binding domain of the arabinose repressor AraR from Bacillus subtilis. Acta Crystallogr D Biol Crystallogr. 2012 Feb;68(Pt 2):176-85. Epub 2012 Jan 17. PMID:22281747 doi:http://dx.doi.org/10.1107/S090744491105414X
