Structural highlights
Publication Abstract from PubMed
The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.
Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide.,Liu B, Garnett JA, Lee WC, Lin J, Salgado P, Taylor J, Xu Y, Lambert S, Cota E, Matthews S Biochem Biophys Res Commun. 2012 Apr 4. PMID:22497887[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Liu B, Garnett JA, Lee WC, Lin J, Salgado P, Taylor J, Xu Y, Lambert S, Cota E, Matthews S. Promoting crystallisation of the Salmonella enteritidis fimbriae 14 pilin SefD using deuterium oxide. Biochem Biophys Res Commun. 2012 Apr 4. PMID:22497887 doi:10.1016/j.bbrc.2012.03.136
