3vsm

Publication Abstract from PubMed

Baculovirus envelope protein ODV-E66 (67-704), in which the N-terminal 66 amino acids are truncated, is a chondroitin lyase. It digests chondroitin and chondroitin 6-sulfate efficiently, but does not digest chondroitin 4-sulfate. This unique characteristic is useful for the preparation of specific chondroitin oligosaccharides and for investigation of the mechanism of baculovirus infection. ODV-E66 (67-704) was crystallized; the crystal diffracted to 1.8 A resolution and belonged to space group P6(2) or P6(4), with unit-cell parameters a = b = 113.5, c = 101.5 A. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.54 A(3) Da(-1).

Crystallization and X-ray diffraction analysis of chondroitin lyase from baculovirus: envelope protein ODV-E66.,Kawaguchi Y, Sugiura N, Onishi M, Kimata K, Kimura M, Kakuta Y Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Feb 1;68(Pt 2):190-2. doi:, 10.1107/S1744309111053164. Epub 2012 Jan 26. PMID:22297996^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.