3wcq

Publication Abstract from PubMed

Cyanidioschyzon merolae (Cm) is a single cell red algae that grows in rather thermophilic (40-50 degrees C) and acidic (pH 1-3) conditions. Ferredoxin (Fd) was purified from this algae and characterized as a plant-type [2Fe-2S] Fd by physicochemical techniques. A high resolution (0.97A) three-dimensional structure of the CmFd D58N mutant molecule has been determined using the Fe-SAD phasing method to clarify the precise position of the Asn58 amide, as this substitution increases the electron-transfer ability relative to wild-type CmFd by a factor of 1.5. The crystal structure reveals an electro-positive surface surrounding Asn58 that may interact with ferredoxin NADP(+) reductase or cytochrome c.

Increasing the electron-transfer ability of Cyanidioschyzon merolae ferredoxin by a one-point mutation - A high resolution and Fe-SAD phasing crystal structure analysis of the Asp58Asn mutant.,Ueno Y, Matsumoto T, Yamano A, Imai T, Morimoto Y Biochem Biophys Res Commun. 2013 Jul 12;436(4):736-9. doi:, 10.1016/j.bbrc.2013.06.029. Epub 2013 Jun 18. PMID:23792094^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.