Structural highlights
Publication Abstract from PubMed
Many viruses regulate translation of polycistronic mRNA using a -1 ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two stems that form a quasi-continuous helix and two connecting loops. A 1.6 A crystal structure of the beet western yellow virus (BWYV) pseudoknot reveals rotation and a bend at the junction of the two stems. A loop base is inserted in the major groove of one stem with quadruple-base interactions. The second loop forms a new minor-groove triplex motif with the other stem, involving 2'-OH and triple-base interactions, as well as sodium ion coordination. Overall, the number of hydrogen bonds stabilizing the tertiary interactions exceeds the number involved in Watson-Crick base pairs. This structure will aid mechanistic analyses of ribosomal frameshifting.
Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot.,Su L, Chen L, Egli M, Berger JM, Rich A Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:10074948[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Su L, Chen L, Egli M, Berger JM, Rich A. Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot. Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:10074948 doi:10.1038/6722
