3j7b

Publication Abstract from PubMed

MicroED is a recently developed method that uses electron diffraction for structure determination from very small three-dimensional crystals of biological material. Previously we used a series of still diffraction patterns to determine the structure of lysozyme at 2.9 A resolution with MicroED (Shi et al., 2013). Here we present the structure of bovine liver catalase determined from a single crystal at 3.2 A resolution by MicroED. The data were collected by continuous rotation of the sample under constant exposure and were processed and refined using standard programs for X-ray crystallography. The ability of MicroED to determine the structure of bovine liver catalase, a protein that has long resisted atomic analysis by traditional electron crystallography, demonstrates the potential of this method for structure determination.

Structure of catalase determined by MicroED.,Nannenga BL, Shi D, Hattne J, Reyes FE, Gonen T Elife. 2014 Oct 10;3:e03600. doi: 10.7554/eLife.03600. PMID:25303172^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.