Structural highlights
Function
[SRYD_DROME] Transcriptional activator that controls bicoid gene expression during oogenesis. Found in transcriptionally active cells. Binds to specific sites on polytene chromosomes of third instar larvae. Binds to the consensus DNA sequence 5'-YTAGAGATGGRAA-3'.[1] [2]
Publication Abstract from PubMed
ZAD is a C4 zinc-coordinating domain often found at the N-terminus mostly of arthropodan transcription factors with multiple C2H2 zinc-finger domains involved in the regulation of chromosome architecture and promotor activity. ZADs predominantly form homodimers and have low primary sequence similarity. We obtained three crystal structures of the most phylogenetically distant Drosophila ZADs and structure of the only known ZAD-like domain from a mammalian protein (ZNF276). All ZAD structures demonstrate unity of the spatial fold as well as some unique structural features. The specific homodimerization of ZAD is primarily determined by the position and size of secondary structural elements and is further strengthened by a number of unique interactions between subunits. Structural comparison allowed for unraveling key sequence features underlying the similarity of the spatial fold. These features result in a broad variety of ZADs in Arthropod C2H2 proteins, allowing for the emergence of a wide range of highly specific homodimers.
Structural insights into highly similar spatial organization of zinc-finger associated domains with a very low sequence similarity.,Bonchuk AN, Boyko KM, Nikolaeva AY, Burtseva AD, Popov VO, Georgiev PG Structure. 2022 Jul 7;30(7):1004-1015.e4. doi: 10.1016/j.str.2022.04.009. Epub, 2022 May 16. PMID:35580610[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Payre F, Buono P, Vanzo N, Vincent A. Two types of zinc fingers are required for dimerization of the serendipity delta transcriptional activator. Mol Cell Biol. 1997 Jun;17(6):3137-45. doi: 10.1128/MCB.17.6.3137. PMID:9154812 doi:http://dx.doi.org/10.1128/MCB.17.6.3137
- ↑ Ruez C, Payre F, Vincent A. Transcriptional control of Drosophila bicoid by Serendipity delta: cooperative binding sites, promoter context, and co-evolution. Mech Dev. 1998 Nov;78(1-2):125-34. doi: 10.1016/s0925-4773(98)00159-2. PMID:9858707 doi:http://dx.doi.org/10.1016/s0925-4773(98)00159-2
- ↑ Bonchuk AN, Boyko KM, Nikolaeva AY, Burtseva AD, Popov VO, Georgiev PG. Structural insights into highly similar spatial organization of zinc-finger associated domains with a very low sequence similarity. Structure. 2022 Jul 7;30(7):1004-1015.e4. doi: 10.1016/j.str.2022.04.009. Epub, 2022 May 16. PMID:35580610 doi:http://dx.doi.org/10.1016/j.str.2022.04.009
