Structural highlights
Publication Abstract from PubMed
We report the engineering of mScarlet, a truly monomeric red fluorescent protein with record brightness, quantum yield (70%) and fluorescence lifetime (3.9 ns). We developed mScarlet starting with a consensus synthetic template and using improved spectroscopic screening techniques; mScarlet's crystal structure reveals a planar and rigidified chromophore. mScarlet outperforms existing red Forster proteins as a fusion tag, and it is especially useful as a fluorescence resonance energy transfer (FRET) acceptor in ratiometric imaging.
mScarlet: a bright monomeric red fluorescent protein for cellular imaging.,Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. PMID:27869816[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr. mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. PMID:27869816 doi:http://dx.doi.org/10.1038/nmeth.4074
