1ixr
From Proteopedia
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| , resolution 3.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | ruvA (Thermus thermophilus), ruvB (Thermus thermophilus) | ||||||
| Activity: | Adenosinetriphosphatase, with EC number 3.6.1.3 | ||||||
| Related: | 1HQC, 1IXS
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RuvA-RuvB complex
Overview
We present the X-ray structure of the RuvA-RuvB complex, which plays a crucial role in ATP-dependent branch migration. Two RuvA tetramers form the symmetric and closed octameric shell, where four RuvA domain IIIs spring out in the two opposite directions to be individually caught by a single RuvB. The binding of domain III deforms the protruding beta hairpin in the N-terminal domain of RuvB and thereby appears to induce a functional and less symmetric RuvB hexameric ring. The model of the RuvA-RuvB junction DNA ternary complex, constructed by fitting the X-ray structure into the averaged electron microscopic images of the RuvA-RuvB junction, appears to be more compatible with the branch migration mode of a fixed RuvA-RuvB interaction than with a rotational interaction mode.
About this Structure
1IXR is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the RuvA-RuvB complex: a structural basis for the Holliday junction migrating motor machinery., Yamada K, Miyata T, Tsuchiya D, Oyama T, Fujiwara Y, Ohnishi T, Iwasaki H, Shinagawa H, Ariyoshi M, Mayanagi K, Morikawa K, Mol Cell. 2002 Sep;10(3):671-81. PMID:12408833
Page seeded by OCA on Sun Mar 30 21:26:09 2008
Categories: Adenosinetriphosphatase | Protein complex | Thermus thermophilus | Ariyoshi, M. | Fujiwara, Y. | Iwasaki, H. | Mayanagi, K. | Miyata, T. | Morikawa, K. | Ohnishi, T. | Oyama, T. | Shinagawa, H. | Tsuchiya, D. | Yamada, K. | Aaa-atpase domain | Complex with nucleotide | Heterooligomeric complex | Octameric ruva
