4nt4

Publication Abstract from PubMed

The CK1 family kinases regulate multiple cellular aspects and play important roles in Wnt/Wingless and Hedgehog signalling. The kinase domain of Drosophila Gilgamesh isoform I (Gilgamesh-I), a homologue of human CK1-gamma, was purified and crystallized. Crystals of methylated Gilgamesh-I kinase domain with a D210A mutation diffracted to 2.85 A resolution and belonged to space group P43212, with unit-cell parameters a = b = 52.025, c = 291.727 A. The structure of Gilgamesh-I kinase domain, which was determined by molecular replacement, has conserved catalytic elements and an active conformation. Structural comparison indicates that an extended loop between the alpha1 helix and the beta4 strand exists in the Gilgamesh-I kinase domain. This extended loop may regulate the activity and function of Gilgamesh-I.

Structure of the kinase domain of Gilgamesh from Drosophila melanogaster.,Han N, Chen C, Shi Z, Cheng D Acta Crystallogr F Struct Biol Commun. 2014 Apr;70(Pt 4):438-43. doi:, 10.1107/S2053230X14004774. Epub 2014 Mar 25. PMID:24699734^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.