1k1s
From Proteopedia
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| , resolution 2.80Å | |||||||
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| Gene: | DINB (Sulfolobus solfataricus) | ||||||
| Related: | 1K1Q
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of DinB from Sulfolobus solfataricus
Overview
A new group of error-prone DNA polymerases overcomes the blockage posed to normal DNA replication by damaged template bases, suggesting an active site with a loose, flexible pocket that accommodates aberrant DNA structures. We have determined a 2.8 A resolution crystal structure of the Sulfolobus solfataricus Dbh protein, a DNA translesion polymerase closely related to Escherichia coli DNA polymerase IV and human polymerase kappa. A high error rate is observed for the Dbh polymerase in a range of 10(-2)-10(-3) for all 12 base substitution mispairs. The crystal structure of Dbh reveals an overall architecture resembling other DNA polymerases but has unique features that are likely to contribute to error-prone synthesis, including -1 frameshifting mutations.
About this Structure
1K1S is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.
Reference
Crystal structure of a DinB family error-prone DNA polymerase from Sulfolobus solfataricus., Silvian LF, Toth EA, Pham P, Goodman MF, Ellenberger T, Nat Struct Biol. 2001 Nov;8(11):984-9. PMID:11685247
Page seeded by OCA on Sun Mar 30 21:42:05 2008
