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1k6d
From Proteopedia
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Acetate CoA-transferase, with EC number 2.8.3.8 | ||||||
| Domains: | PRK09920 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT
Overview
The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the availability of high-quality native and derivatized protein crystals diffracting to high or moderate resolution, respectively. Obtaining such crystals often requires significant effort. The present study demonstrates that phases obtained at low resolution (>3.0 A) from crystals of SeMet-labeled protein can be successfully used for automated structure determination. The crystal structure of acetate CoA-transferase alpha-subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal.
About this Structure
1K6D is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data., Korolev S, Koroleva O, Petterson K, Gu M, Collart F, Dementieva I, Joachimiak A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2116-21. Epub 2002, Nov 23. PMID:12454473
Page seeded by OCA on Sun Mar 30 21:44:02 2008
Categories: Acetate CoA-transferase | Escherichia coli | Single protein | Collart, F. | Dementieva, I. | Joachimiak, A. | Korolev, S. | Koroleva, O. | MCSG, Midwest Center for Structural Genomics. | Petterson, K. | Mcsg | Midwest center for structural genomic | Protein structure initiative | Psi | Structural genomic
