Structural highlights
5b0o is a 12 chain structure with sequence from Salty. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | |
| Related: | 2dpy |
| Gene: | fliI, fla AIII, flaC, STM1972 (SALTY), fliH, fla AII.3, fla BIII, STM1971 (SALTY) |
| Activity: | H(+)-transporting two-sector ATPase, with EC number 3.6.3.14 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[FLII_SALTY] Probable catalytic subunit of a protein translocase for flagellum-specific export, or a proton translocase involved in local circuits at the flagellum. May be involved in a specialized protein export pathway that proceeds without signal peptide cleavage. [FLIH_SALTY] Needed for flagellar regrowth and assembly.
Publication Abstract from PubMed
FliI and FliJ form the FliI6FliJ ATPase complex of the bacterial flagellar export apparatus, a member of the type III secretion system. The FliI6FliJ complex is structurally similar to the alpha3beta3gamma complex of F1-ATPase. The FliH homodimer binds to FliI to connect the ATPase complex to the flagellar base, but the details are unknown. Here we report the structure of the homodimer of a C-terminal fragment of FliH (FliHC2) in complex with FliI. FliHC2shows an unusually asymmetric homodimeric structure that markedly resembles the peripheral stalk of the A/V-type ATPases. The FliHC2-FliI hexamer model reveals that the C-terminal domains of the FliI ATPase face the cell membrane in a way similar to the F/A/V-type ATPases. We discuss the mechanism of flagellar ATPase complex formation and a common origin shared by the type III secretion system and the F/A/V-type ATPases.
Insight into the flagella type III export revealed by the complex structure of the type III ATPase and its regulator.,Imada K, Minamino T, Uchida Y, Kinoshita M, Namba K Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):3633-8. doi:, 10.1073/pnas.1524025113. Epub 2016 Mar 16. PMID:26984495[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Imada K, Minamino T, Uchida Y, Kinoshita M, Namba K. Insight into the flagella type III export revealed by the complex structure of the type III ATPase and its regulator. Proc Natl Acad Sci U S A. 2016 Mar 29;113(13):3633-8. doi:, 10.1073/pnas.1524025113. Epub 2016 Mar 16. PMID:26984495 doi:http://dx.doi.org/10.1073/pnas.1524025113
