| Structural highlights
Function
[VCL2_JUGRE] Seed storage protein.[1] [2] [3] [4]
Publication Abstract from PubMed
Vicilin-buried peptides (VBPs) from edible plants are derived from the N-terminal leader sequences (LSs) of seed storage proteins. VBPs are defined by a common alpha-hairpin fold mediated by conserved CxxxCx(10-14)CxxxC motifs. Here, peanut and walnut VBPs were characterized as potential mediators of both peanut/walnut allergenicity and cross-reactivity despite their low ( approximately 17%) sequence identity. The structures of one peanut (AH1.1) and 3 walnut (JR2.1, JR2.2, JR2.3) VBPs were solved using solution NMR, revealing similar alpha-hairpin structures stabilized by disulfide bonds with high levels of surface similarity. Peptide microarrays identified several peptide sequences primarily on AH1.1 and JR2.1, which were recognized by peanut-, walnut-, and dual-allergic patient IgE, establishing these peanut and walnut VBPs as potential mediators of allergenicity and cross-reactivity. JR2.2 and JR2.3 displayed extreme resilience against endosomal digestion, potentially hindering epitope generation and likely contributing to their reduced allergic potential.
Structure, Immunogenicity, and IgE Cross-Reactivity among Walnut and Peanut Vicilin-Buried Peptides.,Foo ACY, Nesbit JB, Gipson SAY, Cheng H, Bushel P, DeRose EF, Schein CH, Teuber SS, Hurlburt BK, Maleki SJ, Mueller GA J Agric Food Chem. 2022 Feb 9. doi: 10.1021/acs.jafc.1c07225. PMID:35139305[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Teuber SS, Jarvis KC, Dandekar AM, Peterson WR, Ansari AA. Identification and cloning of a complementary DNA encoding a vicilin-like proprotein, jug r 2, from english walnut kernel (Juglans regia), a major food allergen. J Allergy Clin Immunol. 1999 Dec;104(6):1311-20. doi:, 10.1016/s0091-6749(99)70029-1. PMID:10589017 doi:http://dx.doi.org/10.1016/s0091-6749(99)70029-1
- ↑ Maleki SJ, Teuber SS, Cheng H, Chen D, Comstock SS, Ruan S, Schein CH. Computationally predicted IgE epitopes of walnut allergens contribute to cross-reactivity with peanuts. Allergy. 2011 Dec;66(12):1522-9. doi: 10.1111/j.1398-9995.2011.02692.x. Epub 2011, Aug 23. PMID:21883278 doi:http://dx.doi.org/10.1111/j.1398-9995.2011.02692.x
- ↑ Downs ML, Semic-Jusufagic A, Simpson A, Bartra J, Fernandez-Rivas M, Rigby NM, Taylor SL, Baumert JL, Mills EN. Characterization of low molecular weight allergens from English walnut (Juglans regia). J Agric Food Chem. 2014 Dec 3;62(48):11767-75. doi: 10.1021/jf504672m. Epub 2014 , Nov 20. PMID:25388987 doi:http://dx.doi.org/10.1021/jf504672m
- ↑ Dubiela P, Kabasser S, Smargiasso N, Geiselhart S, Bublin M, Hafner C, Mazzucchelli G, Hoffmann-Sommergruber K. Jug r 6 is the allergenic vicilin present in walnut responsible for IgE cross-reactivities to other tree nuts and seeds. Sci Rep. 2018 Jul 27;8(1):11366. doi: 10.1038/s41598-018-29656-4. PMID:30054513 doi:http://dx.doi.org/10.1038/s41598-018-29656-4
- ↑ Foo ACY, Nesbit JB, Gipson SAY, Cheng H, Bushel P, DeRose EF, Schein CH, Teuber SS, Hurlburt BK, Maleki SJ, Mueller GA. Structure, Immunogenicity, and IgE Cross-Reactivity among Walnut and Peanut Vicilin-Buried Peptides. J Agric Food Chem. 2022 Feb 9. doi: 10.1021/acs.jafc.1c07225. PMID:35139305 doi:http://dx.doi.org/10.1021/acs.jafc.1c07225
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