Structural highlights
Publication Abstract from PubMed
[structure: see text] Oligonucleotides with a novel 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized using a novel protecting group strategy for the guanidinium group. This modification enhances the binding affinity of oligonucleotides to RNA as well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE modified oligonucleotides exhibited exceptional resistance to nuclease degradation. The crystal structure of a palindromic duplex formed by a DNA oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A resolution.
2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures.,Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M Org Lett. 2004 Jun 10;6(12):1971-4. PMID:15176796[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M. 2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures. Org Lett. 2004 Jun 10;6(12):1971-4. PMID:15176796 doi:10.1021/ol049470e
