5dz6
From Proteopedia
Acyl transferase from Bacillaene PKS
Structural highlights
Function[PKSC_BACSU] Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the transfer of the malonyl-CoA group to the acyl-carrier-protein AcpK (Mal-AcpK).[1] [2] Publication Abstract from PubMedThe antibiotic bacillaene is biosynthesized in Bacillus subtilis by a hybrid type 1 modular polyketide synthase/nonribosomal peptide synthetase of the trans-acyltransferase (trans-AT) class. Within this system, the essential acyl-group loading activity is provided by the action of three free-standing trans-acting acyltransferases. Here, the recombinant expression, purification and crystallization of the bacillaene synthase trans-acting acyltransferase PksC are reported. A diffraction data set has been collected from a single PksC crystal to 1.44 A resolution and the crystal was found to belong to the orthorhombic space group P2(1)2(1)2(1). Crystallization and preliminary X-ray analysis of the bacillaene synthase trans-acting acyltransferase PksC.,Cuskin F, Solovyova AS, Lewis RJ, Race PR Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Apr 1;67(Pt 4):464-6. doi:, 10.1107/S1744309111003484. Epub 2011 Mar 25. PMID:21505242[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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