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Publication Abstract from PubMed

Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-beta-glucosidase from CAZy family GH1. We further present the three-dimensional structure of the inactivated enzyme, which reveals the constellation of active site residues responsible for the enzyme's specificity and confirms the covalent nature of the inactivation.

Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-beta-glucosidases.,Kwan DH, Jin Y, Jiang J, Chen HM, Kotzler MP, Overkleeft HS, Davies GJ, Withers SG FEBS Lett. 2016 Feb;590(4):461-8. doi: 10.1002/1873-3468.12059. Epub 2016 Feb 14. PMID:26790390^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.