5i4h

Publication Abstract from PubMed

Cathepsin L is a ubiquitously expressed papain-like cysteine protease involved in the endosomal degradation of proteins and has numerous roles in physiological and pathological processes, such as arthritis, osteoporosis, and cancer. Insight into the specificity of cathepsin L is important for elucidating its physiological roles and drug discovery. To study interactions with synthetic ligands, we prepared a presumably inactive mutant and crystallized it. Unexpectedly, the crystal structure determined at 1.4 A revealed that the cathepsin L molecule is cleaved, with the cleaved region trapped in the active site cleft of the neighboring molecule. Hence, the catalytic mutant demonstrated low levels of catalytic activity.

Caught in the act: the crystal structure of cleaved cathepsin L bound to the active site of Cathepsin L.,Sosnowski P, Turk D FEBS Lett. 2016 Apr;590(8):1253-61. doi: 10.1002/1873-3468.12140. Epub 2016 Mar, 30. PMID:26992470^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.