Structural highlights
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A coleopteran triosephosphate isomerase (TIM) from Tenebrio molitor (yellow mealworm beetle) was recombinantly expressed in Escherichia coli and characterized with respect to thermal stability, kinetic parameters and oligomeric state. The enzyme was successfully crystallized and the structure determined by X-ray analysis to 2.0 A resolution. This is the first example of an invertebrate TIM. We compare structural features with known structures of TIMs from microorganisms, plants and vertebrates, and discuss the utility of the Tenebrio TIM sequence, together with several newly sequenced insect TIMs, for molecular phylogenetic analysis.
A coleopteran triosephosphate isomerase: X-ray structure and phylogenetic impact of insect sequences.,Knobeloch D, Schmidt A, Scheerer P, Krauss N, Wessner H, Scholz Ch, Kuttner G, von Rintelen T, Wessel A, Hohne W Insect Mol Biol. 2010 Feb;19(1):35-48. Epub 2009 Oct 22. PMID:19849721[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Knobeloch D, Schmidt A, Scheerer P, Krauss N, Wessner H, Scholz Ch, Kuttner G, von Rintelen T, Wessel A, Hohne W. A coleopteran triosephosphate isomerase: X-ray structure and phylogenetic impact of insect sequences. Insect Mol Biol. 2010 Feb;19(1):35-48. Epub 2009 Oct 22. PMID:19849721 doi:10.1111/j.1365-2583.2009.00928.x
