Structural highlights
Function
Q9ALM7_SACSN
Publication Abstract from PubMed
In the biosynthetic pathway of the spinosyn insecticides, the tailoring enzyme SpnF performs a [4 + 2] cycloaddition on a 22-membered macrolactone to forge an embedded cyclohexene ring. To learn more about this reaction, which could potentially proceed through a Diels-Alder mechanism, we determined the 1.50-A-resolution crystal structure of SpnF bound to S-adenosylhomocysteine. This sets the stage for advanced experimental and computational studies to determine the precise mechanism of SpnF-mediated cyclization.
The structure of SpnF, a standalone enzyme that catalyzes [4 + 2] cycloaddition.,Fage CD, Isiorho EA, Liu Y, Wagner DT, Liu HW, Keatinge-Clay AT Nat Chem Biol. 2015 Mar 2. doi: 10.1038/nchembio.1768. PMID:25730549[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fage CD, Isiorho EA, Liu Y, Wagner DT, Liu HW, Keatinge-Clay AT. The structure of SpnF, a standalone enzyme that catalyzes [4 + 2] cycloaddition. Nat Chem Biol. 2015 Mar 2. doi: 10.1038/nchembio.1768. PMID:25730549 doi:http://dx.doi.org/10.1038/nchembio.1768
