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Publication Abstract from PubMed

Hoogsteen DNA base pairs (bps) are an alternative base pairing to canonical Watson-Crick bps and are thought to play important biochemical roles. Hoogsteen bps have been reported in a handful of X-ray structures of protein-DNA complexes. However, there are several examples of Hoogsteen bps in crystal structures that form Watson-Crick bps when examined under solution conditions. Furthermore, Hoogsteen bps can sometimes be difficult to resolve in DNA:protein complexes by X-ray crystallography due to ambiguous electron density and by solution-state NMR spectroscopy due to size limitations. Here, using infrared spectroscopy, we report the first direct solution-state observation of a Hoogsteen (G-C(+) ) bp in a DNA:protein complex under solution conditions with specific application to DNA-bound TATA-box binding protein. These results support a previous assignment of a G-C(+) Hoogsteen bp in the complex, and indicate that Hoogsteen bps do indeed exist under solution conditions in DNA:protein complexes.

Infrared Spectroscopic Observation of a G-C(+) Hoogsteen Base Pair in the DNA:TATA-Box Binding Protein Complex Under Solution Conditions.,Stelling AL, Liu AY, Zeng W, Salinas R, Schumacher MA, Al-Hashimi HM Angew Chem Int Ed Engl. 2019 Aug 26;58(35):12010-12013. doi:, 10.1002/anie.201902693. Epub 2019 Jul 25. PMID:31268220^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.