Structural highlights
Publication Abstract from PubMed
Cost competitive conversion of biomass-derived sugars into biofuel will require high yields, high volumetric productivities and high titers. Suitable production parameters are hard to achieve in cell-based systems because of the need to maintain life processes. As a result, next-generation biofuel production in engineered microbes has yet to match the stringent cost targets set by petroleum fuels. Removing the constraints imposed by having to maintain cell viability might facilitate improved production metrics. Here, we report a cell-free system in a bioreactor with continuous product removal that produces isobutanol from glucose at a maximum productivity of 4 g L(-1) h(-1), a titer of 275 g L(-1) and 95% yield over the course of nearly 5 days. These production metrics exceed even the highly developed ethanol fermentation process. Our results suggest that moving beyond cells has the potential to expand what is possible for bio-based chemical production.
Isobutanol production freed from biological limits using synthetic biochemistry.,Sherkhanov S, Korman TP, Chan S, Faham S, Liu H, Sawaya MR, Hsu WT, Vikram E, Cheng T, Bowie JU Nat Commun. 2020 Aug 27;11(1):4292. doi: 10.1038/s41467-020-18124-1. PMID:32855421[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sherkhanov S, Korman TP, Chan S, Faham S, Liu H, Sawaya MR, Hsu WT, Vikram E, Cheng T, Bowie JU. Isobutanol production freed from biological limits using synthetic biochemistry. Nat Commun. 2020 Aug 27;11(1):4292. doi: 10.1038/s41467-020-18124-1. PMID:32855421 doi:http://dx.doi.org/10.1038/s41467-020-18124-1
