3e22
From Proteopedia
Tubulin-colchicine-soblidotin: Stathmin-like domain complex
Structural highlights
Function[TBA1C_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). [STMN4_RAT] Exhibits microtubule-destabilizing activity.[1] [2] [3] [TBB2B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe tubulin vinca domain is the target of widely different microtubule inhibitors that interfere with the binding of vinblastine. Although all these ligands inhibit the hydrolysis of GTP, they affect nucleotide exchange to variable extents. The structures of two vinca domain antimitotic peptides--phomopsin A and soblidotin (a dolastatin 10 analogue)--bound to tubulin in a complex with a stathmin-like domain show that their sites partly overlap with that of vinblastine and extend the definition of the vinca domain. The structural data, together with the biochemical results from the ligands we studied, highlight two main contributors in nucleotide exchange: the flexibility of the tubulin subunits' arrangement at their interfaces and the residues in the carboxy-terminal part of the beta-tubulin H6-H7 loop. The structures also highlight common features of the mechanisms by which vinca domain ligands favour curved tubulin assemblies and destabilize microtubules. Structural insight into the inhibition of tubulin by vinca domain peptide ligands.,Cormier A, Marchand M, Ravelli RB, Knossow M, Gigant B EMBO Rep. 2008 Nov;9(11):1101-6. Epub 2008 Sep 12. PMID:18787557[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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Categories: Bos taurus | Buffalo rat | Large Structures | Cormier, A | Gigant, B | Knossow, M | Marchand, M | Ravelli, R B | Alpha-tubulin | Beta-tubulin | Cell cycle | Colchicine | Gtpase | Microtubule | Soblidotin | Stathmin | Tubulin
