Structural highlights
Function
[PLDH_RHILO] Involved in the degradation of pyridoxine or pyridoxamine (free, phosphate-unbound, forms of vitamin B6). Oxidizes pyridoxal to 4-pyridoxolactone, but does not have activity toward pyridoxal 5'-phosphate, pyridoxine, pyridoxamine, pyridoxamine 5'-phosphate, 4-phthalaldehyde, 2-nitrobenzaldehyde, pyridine, formaldehyde, 2-carboxybenzaldehyde or sugars.[1]
Publication Abstract from PubMed
Pyridoxal 4-dehydrogenase catalyzes the irreversible oxidation of pyridoxal to 4-pyridoxolactone and is involved in degradation pathway I of pyridoxine, a vitamin B(6) compound. Its crystal structure was elucidated for the first time. Molecular replacement with (S)-1-phenylthanol dehydrogenase (PDB code 2EW8) was adopted to determine the tertiary structure of the NAD(+)-bound enzyme.
The crystal structure of SDR-type pyridoxal 4-dehydrogenase of Mesorhizobium loti.,Chu HN, Kobayashi J, Mikami B, Yagi T Biosci Biotechnol Biochem. 2011;75(2):388-90. Epub 2011 Feb 7. PMID:21307574[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yokochi N, Nishimura S, Yoshikane Y, Ohnishi K, Yagi T. Identification of a new tetrameric pyridoxal 4-dehydrogenase as the second enzyme in the degradation pathway for pyridoxine in a nitrogen-fixing symbiotic bacterium, Mesorhizobium loti. Arch Biochem Biophys. 2006 Aug 1;452(1):1-8. Epub 2006 Jun 16. PMID:16824480 doi:http://dx.doi.org/10.1016/j.abb.2006.06.002
- ↑ Chu HN, Kobayashi J, Mikami B, Yagi T. The crystal structure of SDR-type pyridoxal 4-dehydrogenase of Mesorhizobium loti. Biosci Biotechnol Biochem. 2011;75(2):388-90. Epub 2011 Feb 7. PMID:21307574
