3u55

Publication Abstract from PubMed

The first native crystal structure of Phosphoribosylaminoimidazole-succinocarboxamide synthetase (SAICAR synthetase) from a hyperthermophilic organism Pyrococcus horikoshii OT3 was determined in two space groups H3 (Type-1: Resolution 2.35A) and in C222(1) (Type-2: Resolution 1.9A). Both are dimeric but Type-1 structure exhibited hexameric arrangement due to the presence of cadmium ions. A comparison has been made on the sequence and structures of all SAICAR synthetases to better understand the differences between mesophilic, thermophilic and hyperthermophilic SAICAR synthetases. These SAICAR synthetases are reasonably similar in sequence and three-dimensional structure; however, differences were visible only in the subtler details of percentage composition of the sequences, salt bridge interactions and non-polar contact areas.

Structure of SAICAR synthetase from Pyrococcus horikoshii OT3: insights into thermal stability.,Manjunath K, Kanaujia SP, Kanagaraj S, Jeyakanthan J, Sekar K Int J Biol Macromol. 2013 Feb;53:7-19. doi: 10.1016/j.ijbiomac.2012.10.028. Epub , 2012 Nov 5. PMID:23137517^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.